Weeks 6 and 7 were very similar. Kevin and I would observe the 6 tanks, each with 3 fish, for 3 hours. Then, we would remove the fish from the spawning tanks, weigh them, and put each fish back to their respective tank. Afterwards, Kevin went to the lab's office and enter our data for the day onto the computer. Simultaneously, I would remove the eggs from the spawning tanks, count the fertilized, unfertilized, and nonviable eggs in each tank, and set the eggs in the incubation tanks where they would grow for 24 hours.
The 24 hour mark is important to the experiment. After 24 hours, we are able to determine which cells were the result of the triploid or diploid male. This is because the DNA in the diploid zebrafish male's were altered to make the fish transparent. This specific type of zebrafish is called a "casper." As a result, the eggs laid by the casper zebrafish are also transparent. Due to this, we are able to determine the fertilization rate of each fish.
The fish to the left is a wild-type zebrafish, where as the fish to the right is an example of a casper zebrafish.
Kevin did a great job entering all of the data into the computer!