On August 27, instead of doing my usual 3 hours of zebrafish observations, I began my day helping with an in vitro fertilization. The Professor decided to discontinue the 3 hour observations because we had gathered enough information from them. Now, it was necessary to interpret out results and decide the next path to take.
To perform multiple in vitro fertilizations, it would be necessary to take the sperm from multiple different males; however, this is extremely inefficient. Instead, I took the testes from one male zebrafish, macerated the sample, and centrifuged the solution. Because of this, I had enough sperm for multiple fertilizations.
After I finished the in vitro fertilization, I helped feed the fish. The zebrafish's diet is brine shrimp (The orange solution).